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bme (R&D Systems)

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R&D Systems bme
Bme, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bme/Cultrex+ReadyBME%2C+Stem+Cell+Qualified%2C+Reduced+Growth+Factor/pm42031998-58-6-7
Average 93 stars, based on 6 article reviews

bme - by Bioz Stars, 2026-09

93/100 stars

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Single Cell:

Article Title: Interferon-γ selectively promotes survival of alveolar progenitor cells in a human lung organoid model.
Article Snippet: PneumaCultTM Alveolar Organoid Expansion Media (STEMCELL Technologies #100-0847) and PneumaCultTM Alveolar Organoid Differentiation Medium (STEMCELL Technologies #100-0861) were used as outlined in the results section. .. Transwell inserts (Greiner bio-one #662641) were coated with 10 μg/ml Invasin (Wijnakker et al, 2025) in PBS or 2% Collagen I © The Author(s) The EMBO Journal 17 (Advanced Biomatrix #5074) in washing media (Table EV1) at 4 °C overnight, or with 5% BME (R&D Systems #3533-005-02) in washing media (Table EV1) at 37 °C for 30 min. Day 14 organoids cultures were processed to single-cell suspensions as outlined in “Making single-cell suspensions and passaging organoids” and 100k–200k cells were plated per transwell. ..

Article Title: Interferon-γ selectively promotes survival of alveolar progenitor cells in a human lung organoid model.
Article Snippet: .. Cells were plated in a mixture of 20% washing buffer (Table EV1) and 80% BME (R&D Systems #3533-005-02) at 300–400 cells/ul as outlined in “Making single-cell suspensions and passaging organoids”. ..

Passaging:

Concentration Assay:

Article Title: Interferon-γ selectively promotes survival of alveolar progenitor cells in a human lung organoid model.
Article Snippet: .. When no BME was visible above the cell pellet anymore, the cells were resuspended in a mixture of 20% washing buffer (Table EV1) and 80% BME (R&D Systems #3533-005-02) at a concentration of 300–400 cells/μl. .. Drops of 10–25 μl were plated on pre-warmed suspension culture plates (Greiner bio-one #657185, #662102, #665102).

Cell Culture:

Article Title: Modeling herpes simplex virus type 2 and Zika virus replication in vaginal organoids and spheroids
Article Snippet: .. For organoid cultures, 20,000 cells were resuspended in 50 μl BME (R&D Systems Cat No. 3533-010-02) and cultured in DMEM/F12 supplemented with 2% Ultroser G (Sartorius Cat No. 15950-017), 1% PS, 50 ng/ml mouse EGF (Sigma Cat No. SRP3196-500UG), 0.5 mM SB431542 (Selleck Chemicals Cat No. S1067), and 10 mM Y-27632 (Tocris Cat No. 1254) (OCM). ..